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氨基酸的主要生產方法有發酵法、酶法、蛋白質水解提取法和化學合成法。目前多采用發酵方式。然而,由于羥脯氨酸的特殊結構,不能產生發酵法,而且化學合成法的成本過高,因此從膠原蛋白中分離和提取是一種重要的方法。蛋白質被酸、堿或蛋白質溶解酶水解,然后各種氨基酸分離純化,簡稱水解法。主要有酸水解、堿水解和酶水解三種水解方法。堿方法可引起氨基酸的外消旋。酶法的缺點是不完全水解。它們很少用于氨基酸的生產。因此,酸水解一般用于制備羥脯氨酸。

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The main production methods of amino acids are fermentation, enzymatic method, protein hydrolysis extraction method and chemical synthesis method. At present, fermentation method is mostly used. However, because of the special structure of hydroxyproline, fermentation method can not be produced, and the cost of chemical synthesis method is too high, so isolation and extraction from collagen is an important way. Protein is hydrolyzed by acid, alkali or proteolytic enzymes, and then various amino acids are separated and purified, which is called hydrolysis method for short. There are three main hydrolysis methods: acid hydrolysis, alkali hydrolysis and enzymatic hydrolysis. Alkali method can cause racemization of amino acids. The disadvantage of enzymatic method is incomplete hydrolysis. Intermediate products such as peptides are abundant, the general time is long, and the bacteria are easily polluted. They are seldom used in the production of amino acids. Therefore, acid hydrolysis is generally used to prepare hydroxyproline.

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